Growing environmental concerns have led stakeholders to scrutinise business activities and products that pose significant ecological risks. Increasing pressure from international consumers and regulatory authorities has urged companies to adopt environmentally sustainable strategies to reduce their ecological footprint. In response to these expectations, firms are encouraged to integrate sustainable practices, consequently driving greater investment in the Green Supply Chain (GSC). This study investigates the impact of Green Supply Chain Practices (GSCP) on Firm Performance (FP) within Pakistan’s textile sector. It further assesses the mediating role of Supply Chain Environmental Concern (SCEC) and the moderating influence of Organisational Culture (OC) in this relationship. A quantitative methodology was adopted, drawing upon primary data from 120 supply chain managers in the Pakistani textile industry. The dataset was analysed using Smart-PLS 3. The findings demonstrate a significant and positive influence of GSCP on FP. Moreover, SCEC significantly mediates the relationship between GSCP and FP. In addition, OC was found to moderate the link between SCEC and FP. These results indicate that GSCP and SCEC positively enhance FP, with OC strengthening this effect.